Surfacine kills microorganisms on contact - but is non-toxic to mammalian cells.
In laboratory testing, Surfacine treated surfaces effectively eliminated all human pathogens tested - including bacteria, yeasts and fungi. Typical testing protocol involves bacterial challenges of 10^6 CFU/mL (colony forming units per milliliter of saline) inoculation on treated and untreated control surfaces. After 20 hours in 25C humidity chamber, the surfaces were swabbed and the live bacteria were counted using the conventional plate counting method. Control surfaces show bacteria counts of 10^4 to 10^6 CFU/mL while Surfacine treated surfaces show no measurable level of living microorganisms. We utilized contract laboratories to perform mold and virus testing according to standard protocols. To date, Surfacine proved efficacious against all pathogens tested in this method. The following table lists all the pathogens tested to date, their ATCC strain number, type of organism, and the type of efficacy tested:
| Pathogen | ATCC# | Type | Efficacy |
| Escherichia coli | 8739 | Gram negative bacteria | Sustained |
| Escherichia coli O157:H7 | 43895 | Gram negative bacteria | Immediate & sustained |
| Pseudomonas aeruginosa | 9027 | Gram negative bacteria | Immediate & sustained |
| Pseudomonas aeruginosa | 15442 | Gram negative bacteria | Immediate & sustained |
| Pseudomonas cepacia | 25416 | Gram negative bacteria | Immediate & sustained |
| Pseudomonas diminuta | 19146 | Gram negative bacteria | Immediate & sustained |
| Klebsiella pneumoniae | 13883 | Gram negative bacteria | Sustained |
| Serratia marcescens | 8100 | Gram negative bacteria | Sustained |
| Brevundimonas diminuta | 19146 | Gram negative bacteria | Sustained |
| Burkholderia cepacia | 25416 | Gram negative bacteria | Sustained |
| Campylobacter jejuni | 33560 | Gram negative bacteria | Sustained |
| Escherichia coli | 11229 | Gram negative bacteria | Immediate & sustained |
| Escherichia coli | 10536 | Gram negative bacteria | Immediate & sustained |
| Escherichia coli | 11775 | Gram negative bacteria | Sustained |
| Escherichia coli | 4157 | Gram negative bacteria | Sustained |
| Myroides odoratus | 4651 | Gram negative bacteria | Sustained |
| Providencia alcalifaciens | 9886 | Gram negative bacteria | Sustained |
| Shigella flexneri | 29903 | Gram negative bacteria | Sustained |
| Enterococcus faecalis | 19433 | Gram positive bacteria | Sustained |
| Streptococcus mutans | 35668 | Gram positive bacteria | Immediate & sustained |
| Streptococcus sanguis | 10556 | Gram positive bacteria | Sustained |
| Staphylococcus aureus | 6538 | Gram positive bacteria | Immediate & sustained |
| Methicillin resistant Staphylococcus aureus | 33592 | Gram positive bacteria | Sustained |
| Staphylococcus epidermidis | 12228 | Gram positive bacteria | Immediate & sustained |
| Bacillus cereus | 11778 | Gram positive bacteria | Sustained |
| Salmonella choleraesuis | 10708 | Gram negative bacteria | Immediate & sustained |
| Bacillus subtilis | 6633 | Gram positive bacteria | Sustained |
| Bacillus subtilis | 19659 | Gram positive bacteria | Immediate |
| Enterococcus faecalis (Vancomycin resistant) | 51559 | Gram positive bacteria | Immediate & sustained |
| Enterococcus faecium (MDR) | 51559 | Gram positive bacteria | Sustained |
| Listeria monocytogenes | 19114 | Gram positive bacteria | Sustained |
| Listeria monocytogenes | 15313 | Gram positive bacteria | Sustained |
| Aspergillus niger (Example) | 9642 | Mold | Sustained |
| Aspergillus niger (static) (Example) | 6275 | Mold | Immediate & sustained |
| Candida albicans | 18804 | Yeast | Immediate & sustained |
| Candida parabsilosis | 20246 | Yeast | Immediate & sustained |
| Saccharomyces cerevisiae | 834 | Yeast | Sustained |
| Trichophyton mentagrophytes | 9533 | Mold | Immediate |
| Hepatitis A | VR-1073 | Virus | Immediate |
| Candida albicans | 10231 | Yeast | Immediate & sustained |
| Herpes Simplex Type 1 | VR-733 | Virus | Immediate |
| Polio Virus Type 1 | VR-1000 | Virus | Immediate |
| Rhinovirus Type 1 | VR-1147 | Virus | Immediate |