Surfacine remains fully inert and bio-active after exposure to a variety of physical and chemical stresses:

• Low temperature (-15°C)
• Ethanol and boiling water
• Prolonged exposure to solutions of varying pH (4 - 10)
• High ionic strength solution (2% sodium chloride)
• Long term exposure to urine (35°C for 7 days)
• Human serum albumin (0.7%) in phosphate buffered saline
• Exposure to blood products
• Worn by human volunteers for a 3 day period
• Gamma irradiation (5 Mrad)
• E-beam sterilization (25 kGrays)
• Ethylene oxide sterilization
• Autoclave (wet or dry)
• Multiple exposure to bacterial challenge